Fig. 6.
Inactivation of MEK blocks ARF6-GTP-induced cell invasion. (A and B) ARF6(Q67L)-transfected cells were seeded on CFDSE-labeled, gelatin-coated coverslips and allowed to invade in the presence of the MEK inhibitor, PD98059. Cells were fixed, permeablized, and immunofluorescently labeled for HA. A and B are taken along the x/y and x/z axis respectively. (Bar = 10 μm.) (C) LOX cells were singly or dually transfected with plasmids encoding ARF6 and MEK-1 mutants as indicated. Cells were seeded on gelatin and treated with or without PD98059 as indicated. The percentage of single or dually transfected cells exhibiting gelatin degradation underneath them was scored.