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. 2015 Dec 11;196(2):891–901. doi: 10.4049/jimmunol.1501648

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Copyright © 2016 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 4. — The numbers of lymphocytes in tumor-bearing lungs are decreased in the setting of 5-LO-KO in the TME. (A) Sequential flow cytometry gating strategy to identify lymphocytes in tumor-bearing lungs: NK cells (NK 1.1⁺/CD3⁻), CD3 cells (CD3⁺/SSC^Low), CD4 cells (CD3⁺/CD4⁺), and CD8 cells (CD3⁺/CD8⁺). (B) Flow cytometry–based quantitation of CD3, CD4, and CD8 cells in tumor bearing lungs from WT and 5-LO-KO mice, harvested 2.5 wk after LLC-Luc cells injection. Each dot represents a single mouse. Data represent three separate flow analyses. (C) Quantitation of NK cells and dendritic cells (DC). DC were identified using myeloid marker panel (CD11c^Hi/MHC class II^Hi/SigF⁻). (D) Number of CD3-positive cells was assessed by immunohistochemistry. Quantification was performed in sections from six WT and four 5-LO-KO mice.