Figure 3.
Senescence markers are decreased in very old CIGF1RKO hearts. A, Sections were stained with SA-β-gal. Representative images are shown (magnification, ×40; scale bars, 30 μm). B, SA-β-gal-positive cells (arrows) were quantified as the number of cells positive for SA-β-gal divided by the number of nuclei. Numbers of mice are indicated on the bars. C, mRNA quantification of cell cycle inhibitors. Results were normalized to actin and mRNA levels in WT hearts were arbitrarily set as 1 (n = 4–6 per group). Data are presented as the mean ± SEM. Two-way ANOVA was performed to analyze differences by age (young vs very old) and genotype (WT vs CIGF1RKO). *, P < .05; **, P < .01; ***, P < .001 vs young WT or young CIGF1RKO; ###, P < .001 vs very old WT. VO, very old; Y, young.