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. 2015 Dec 7;126(1):220–238. doi: 10.1172/JCI79380

Figure 8. The adenosine A1R antagonist DPCPX decreases cortical F-actin.

Figure 8

(A) Adenosine receptor mRNA expression by real-time qPCR. (B) Representative immunoblot of total actin isolated from confluent HEC-1-A cells treated with DMSO (0.001%), 10 μM NECA, 10 μM NECA plus the A1R antagonist DPCPX (10 μM) or plus the A2BR antagonist MRS1754 (10 μM) in hypoxia (1% O2, 5% CO2) for 36 hours. Graph shows densitometric data from 5 independent experiments. (C) HEC-1-A cells labeled with Alexa Fluor–conjugated phalloidin. (D) F-actin intensity was assessed in 10 or more images (original magnification, ×20). Data represent the mean ± SEM. **P < 0.005 compared with DMSO; *P < 0.05 compared with NECA; and #P < 0.001 compared with NECA plus MRS1754; 1-way ANOVA with Tukey’s post test. Scale bars: 20 μm.