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Effect of IH on phosphorylated PLCγ, PKC, and mTOR levels in the brain cortex, brain stem, and cerebellum of mice. Adult male BALB/c mice were exposed to normoxia (N) or IH (8 h/day × 10 days). Levels of phospho-PLCγ (p-PLCγ), total PLCγ (PLCγ), phospho-PKC (p-PKC), total PKC (PKC), phosphorylated mTOR (p-mTOR), and total mTOR (mTOR) were determined by immunoblot assays of tissue lysates prepared from cerebral cortex, brain stem, and cerebellum.
