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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Immunity. 2015 Dec 15;43(6):1125–1136. doi: 10.1016/j.immuni.2015.11.016

Figure 5. The IL-1α-deficient mice develop non-resolving monocytic inflammation.

Figure 5

(A) The weight of lungs harvested from WT and Il1a−/− mice at 90 days after Mtb infection. N = 30, ** P < 0.01.

(B) Total number of mononuclear cells purified from lungs of WT are Il1a−/− mice at indicated times after Mtb infection. N = 5. * P < 0.05; ** P < 0.01.

(C) Flow cytometry dot plots of the distribution of Ly-6C-and Ly-6G-expressing cell subsets in the lungs of WT and Il1a−/− mice 90 days after Mtb infection. The average percentage of Ly-6C+ or Ly-6C+Ly-6G+ populations are shown for each strain. N=4.

(D) Analysis of Ly-6C expression on CD11b- and CD11c-expressing subsets of mononuclear cells purified from the lungs of Mtb-infected WT and Il1a−/− mice. Median intensity of Ly-6C staining on CD11b+ and CD11c+ (population b) subsets are shown in histogram plots on the right. N=4. a and b designate individual Ly-6C+ cell populations expressing different amounts of CD11c.

(E) Analysis of CD40-expressing CD11c+ cell subsets in the lungs of WT and Il1a−/− mice after Mtb infection. The average percentage of CD11c+CD40high (population a) and CD11chighCD40low (population b) subset are shown. N=4.

(F) Flow cytometry dot plots of CD11c+CD40+ cells in the lungs of bone marrow chimeric WT mice, transplanted with either WT or Il1a−/− bone marrow cells. Recipient WT mice were lethally irradiated and transplanted with bone marrow cells from either WT or Il1a−/− mice. Five months after the bone marrow transplantation, mice were infected with Mtb and indicated cell subsets were analyzed in the lungs 80 days after infection. Average percentage of CD11c+CD40high and CD11chighCD40low are shown. N=3.