Table 2. Pearson correlation coefficients and adjusted p values (r;p) between the significantly correlated molecular and histological parameters for the nine samples analysed in the testicular miRNA profiling.
| TESE-value | Elongated spermatids/tubule | AUC | 95% CI | p-value | |
|---|---|---|---|---|---|
| hsa-let-7b* | −0,703;p = 0,035 | −0,719;p = 0,029 | 0,950 | 0,806–1,094 | 0,027 |
| hsa-miR-106b* | 0,718;p = 0,029 | 0,692;p = 0,039 | 0,250 | −0,090–0,590 | 0,221 |
| hsa-miR-10b* | −0,884;p = 0,002 | −0,879;p = 0,002 | 1,000 | 1,000–1,000 | 0,014 |
| hsa-miR-122 | 0,948;p = 0,0001 | 0,975;p < 0,0001 | 0,000 | 0,000–0,000 | 0,014 |
| hsa-miR-124 | 0,904;p = 0,002 | 0,880;p = 0,004 | 0,125 | −0,142–0,392 | 0,083 |
| hsa-miR-148* | −0,845;p = 0,008 | −0,842;p = 0,009 | 0,750 | 0,326–1,174 | 0,248 |
| hsa-miR-15b | 0,805;p = 0,009 | 0,833;p = 0,005 | 0,100 | −0,121–0,321 | 0,05 |
| hsa-miR-16-2* | 0,879;p = 0,002 | 0,885;p = 0,002 | 0,100 | −0,121–0,321 | 0,05 |
| hsa-miR-182 | 0,951;p < 0,0001 | 0,970;p < 0,0001 | 0,100 | −0,121–0,321 | 0,05 |
| hsa-miR-194 | 0,828;p = 0,006 | 0,839;p = 0,005 | 0,150 | −0,112–0,412 | 0,086 |
| hsa-miR-202* | −0,814;p = 0,008 | −0,800;p = 0,010 | 1,000 | 1,000–1,000 | 0,014 |
| hsa-miR-210 | −0,793;p = 0,011 | −0,788;p = 0,012 | 0,950 | 0,806–1,094 | 0,027 |
| hsa-miR-215 | 0,744;p = 0,022 | 0,738;p = 0,023 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-23a* | 0,854;p = 0,007 | 0,815;p = 0,014 | 0,000 | 0,000–0,000 | 0,025 |
| hsa-miR-26a | −0,696;p = 0,037 | −0,692;p = 0,039 | 0,900 | 0,679–1,121 | 0,05 |
| hsa-miR-26a-2* | −0,721;p = 0,028 | −0,715;p = 0,030 | 1,000 | 1,000–1,000 | 0,014 |
| hsa-miR-27b* | −0,786;p = 0,036 | −0,759;p = 0,048 | 1,000 | 1,000–1,000 | 0,034 |
| hsa-miR-296-3p | 0,834;p = 0,005 | 0,868;p = 0,002 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-296-5p | 0,733;p = 0,025 | 0,733;p = 0,025 | 0,150 | −0,142–0,442 | 0,086 |
| hsa-miR-30e* | 0,761;p = 0,017 | 0,804;p = 0,009 | 0,200 | −0,119–0,510 | 0,142 |
| hsa-miR-31* | 0,755;p = 0,030 | 0,711;p = 0,048 | 0,125 | −0,142–0,392 | 0,083 |
| hsa-miR-330-5p | −0,761;p = 0,028 | −0,756;p = 0,031 | 0,750 | 0,326–1,174 | 0,248 |
| hsa-miR-340* | 0,749;p = 0,032 | 0,740;p = 0,036 | 0,000 | 0,000–0,000 | 0,025 |
| hsa-miR-342-3p | −0,703;p = 0,035 | −0,667;p = 0,050 | 0,700 | 0,319–1,081 | 0,327 |
| hsa-miR-34b | 0,921;p = 0,0004 | 0,931;p = 0,0002 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-34b* | 0,882;p = 0,002 | 0,899;p = 0,001 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-34c–5p | 0,862;p = 0,003 | 0,883;p = 0,002 | 0,050 | −0,094-0,194 | 0,027 |
| hsa-miR-375 | 0,787;p = 0,012 | 0,815;p = 0,007 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-449a | 0,890;p = 0,001 | 0,915;p = 0,001 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-449b | 0,836;p = 0,005 | 0,845;p = 0,004 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-449b* | 0,807;p = 0,028 | 0,779;p = 0,039 | 0,000 | 0,000–0,000 | 0,034 |
| hsa-miR-488 | 0,776;p = 0,014 | 0,784;p = 0,012 | 0,100 | −0,121–0,321 | 0,05 |
| hsa-miR-499-5p | 0,766;p = 0,027 | 0,728;p = 0,041 | 0,188 | −0,160–0,535 | 0,149 |
| hsa-miR-502-3p | −0,703;p = 0,035 | −0,726;p = 0,027 | 0,900 | 0,679–1,121 | 0,05 |
| hsa-miR-504 | −0.955;p = 0,001 | −0,950;p = 0,001 | 0,833 | 0,500–1,167 | 0,157 |
| hsa-miR-511 | 0,984;p < 0,0001 | 0,969;p < 0,0001 | 0,000 | 0,000–0,000 | 0,025 |
| hsa-miR-512-5p | 0,719;p = 0,029 | 0,713;p = 0,030 | 0,000 | 0,000–0,000 | 0,014 |
| hsa-miR-516a-5p | 0,695;p = 0,038 | 0,678;p = 0,045 | 0,000 | 0,000–0,000 | 0,014 |
| hsa-miR-516b | 0,999;p < 0,0001 | 0,999;p < 0,0001 | 0,000 | 0,000–0,000 | 0,025 |
| hsa-miR-517a | 0,672;p = 0,047 | 0,691;p = 0,039 | 0,150 | −0,112–0,412 | 0,086 |
| hsa-miR-517b | 0,757;p = 0,018 | 0,744;p = 0,021 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-517c | 0,854;p = 0,003 | 0,860;p = 0,003 | 0,000 | 0,000–0,000 | 0,014 |
| hsa-miR-518a-3p | 0,754;p = 0,019 | 0,781;p = 0,013 | 0,150 | 0,137–0,437 | 0,086 |
| hsa-miR-518e | 0,805;p = 0,009 | 0,781;p = 0,013 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-518e* | 0,719;p = 0,045 | 0,714;p = 0,047 | 0,067 | −0,118–0,251 | 0,053 |
| hsa-miR-518f | 0,759;p = 0,018 | 0,761;p = 0,017 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-519a | 0,784;p = 0,012 | 0,773;p = 0,015 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-519c-3p | 0,857;p = 0,003 | 0,875;p = 0,002 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-520a-3p | 0,736;p = 0,024 | 0,779;p = 0,013 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-520a-5p | 0,767;p = 0,016 | 0,771;p = 0,015 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-520d-5p | 0,832;p = 0,040 | 0,828;p = 0,042 | 0,000 | 0,000–0,000 | 0,064 |
| hsa-miR-521 | 0,933;p = 0,0002 | 0,950;p < 0,0001 | 0,000 | 0,000–0,000 | 0,014 |
| hsa-miR-525-5p | 0,776;p = 0,024 | 0,808;p = 0,015 | 0,067 | −0,118–0,251 | 0,053 |
| hsa-miR-532-3p | −0,704;p = 0,034 | −0,717;p = 0,030 | 0,900 | 0,679–1,121 | 0,05 |
| hsa-miR-548o | −0,770;p = 0,015 | −0,767;p = 0,016 | 0,850 | 0,558–1,142 | 0,086 |
| hsa-miR-551a | −0,825;p = 0,012 | −0,822;p = 0,012 | 0,875 | 0,608–1,142 | 0,083 |
| hsa-miR-551b | 0,828;p = 0,011 | 0,854;p = 0,007 | 0,188 | −0,126–0,501 | 0,149 |
| hsa-miR-566 | 0,879;p = 0,009 | 0,880;p = 0,009 | 0,083 | −0,143–0,309 | 0,077 |
| hsa-miR-625* | 0,973;p < 0,0001 | 0,954;p < 0,0001 | 0,100 | −0,121–0,321 | 0,05 |
| hsa-miR-629 | 0,847;p = 0,004 | 0,834;p = 0,005 | 0,250 | −0,174–0,674 | 0,221 |
| hsa-miR-643 | 0,914;p = 0,002 | 0,929;p = 0,001 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-769-5p | 0,753;p = 0,019 | 0,728;p = 0,026 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-885-5p | 0,884;p = 0,002 | 0,901;p = 0,001 | 0,100 | −0,121–0,321 | 0,05 |
| hsa-miR-888 | 0,991;p < 0,0001 | 0,984;p < 0,0001 | 0,000 | 0,000–0,000 | 0,025 |
| hsa-miR-9 | 0,741;p = 0,022 | 0,802;p = 0,009 | 0,100 | −0,119–0,319 | 0,05 |
| hsa-miR-96 | 0,926;p = 0,0003 | 0,956;p < 0,0001 | 0,050 | −0,094–0,194 | 0,027 |
| hsa-miR-99b* | −0,737;p = 0,024 | −0,744;p = 0,021 | 0,850 | 0,558–1,142 | 0,086 |
| hsa-miR-505* | −0,882;p = 0,020 | −0,877;p = 0,022 | 1,000 | 1,000–1,000 | 0,064 |
ROC analysis showing the predictive efficiency of miRNA expression values for determining the presence of intratesticular mature sperm cells (TESE value > 0,002; elongated spermatids/tubule > 0,1).
Only those miRNAs that presented significant correlation coefficients for both TESE value and elongated spermatids/tubules are indicated. Those miRNAs that resulted in good predictive accuracy (AUC > 0,900 for those negatively correlated and AUC < 0,05 for those positively correlated; p ≤ 0,03) after the ROC analysis are depicted in bold and italics.
TESE value is defined as the number of spermatozoa that were obtained directly after processing 100 mg of biopsy in 1 mL of medium.