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. Author manuscript; available in PMC: 2016 Nov 17.
Published in final edited form as: Nat Commun. 2015 Nov 17;6:8852. doi: 10.1038/ncomms9852

Figure 2. The VAMP2 mutations do not interfere with the binding of Munc18-1 to the fully assembled cis-SNARE complex.

Figure 2

(a) Schematic illustrations of the cis- and trans-SNARE configurations. SNARE complexes were modelled from atomic structures35. (b) Ternary SNARE complexes were composed of t-SNARE CDs (syntaxin-1 (a.a. 1–265) and full-length SNAP-25) and VAMP2 CD (a.a. 1–95, WTor mutant). The VAMP2 mutants are illustrated in Fig. 1a. Munc18-1 (5 μM) was loaded into the sample cell of the VP-ITC instrument, followed by the injection of SNARE complexes (75 μM) into the sample cell. The dissociation constant (Kd) and stoichiometry (n) of the interaction were calculated by fitting the data with a nonlinear least squares routine using the Microcal Origin software.