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. Author manuscript; available in PMC: 2015 Dec 2.
Published in final edited form as: Biochem J. 2012 Aug 15;446(1):99–111. doi: 10.1042/BJ20120637

Figure 2. Distribution of LRRK2 in cellular compartements.

Figure 2

(A) Full-length WT and G2385R 3xFlag-LRRK2 as well as C-terminus truncated forms of the protein are predominantly associated with microsomal-enriched fraction when transiently expressed in HEK293FT cells. (B, C) Endogenous LRRK2 was mostly found in microsomal-enriched fraction of lymphoblastoid cells or whole brain homogenates of 2 month old mouse (FVB/N) using MJF2 antibody. The following subcellular markers were used for fraction identification: PDI for endoplasmic reticulum or γ-adaptin for trans-Golgi (both second panels), MEK1/2 for cytosol (third panels), and Tom 20 for mitochondria (bottom panels). Each fractionation is representative of triplicate experiments performed at different times.