Figure 3.
Effect of MCP-1 on the migration/invasion of colorectal cancer cells. A and B: HCT 116 cells were treated with MCP-1 (0, 5, 10 or 20 ng/ml) for overnight and assayed for cell migration (A) and invasion (B). C: HCT116 cells were pretreated either with DMSO (control) or CCR2 antagonist (20 μM) for 2 hours and then exposed to MCP-1 (10 ng/ml) overnight. After that, cell migration was assayed as described above. D: HCT116 cells were pretreated either with DMSO (control) or CCR2 antagonist (20 μM) for 2 hours and exposed to alcohol (43.4 mM) overnight, then assayed for cell migration. Each data point was the mean ± SEM of three independent experiments and presented relative to the controls. * denotes significant difference from control groups. # denotes significant difference from MCP-1- or alcohol-treated groups.