Table 2.
Guanosine had no effect on cellular viability
| Treatment | PI incorporation (% of control) | MTT (% of control) |
|---|---|---|
| LPS | 95 ± 8 | 96 ± 8 |
| GUO | 100 ± 8 | 101 ± 8 |
| GUO + LPS | 107 ± 8 | 98 ± 7 |
Cells were incubated in serum-free DMEM/F12 with 100 μM guanosine (GUO) for 1 h, followed by the addition of 10 μg/mL LPS for 3 h. The cells were co-incubated with 10 μM ZnPP IX along with the guanosine. PI incorporation was measured and MTT assay was conducted as described in the “Materials and methods” section. Data represent the mean ± S.E.M. of three independent experiments that were performed in triplicate. Differences between groups were statistically analyzed using two-way ANOVA, followed by Tukey’s test. Values of P < 0.05 were considered significant