. 2015 Oct 2;11(4):571–580. doi: 10.1007/s11302-015-9475-2

Table 1.

The effects of guanosine on LPS-induced inflammatory response were independent of the adenosinergic system

Treatment	TNF-α levels (% of control)	IL-1β levels (% of control)
LPS	280 ± 18^a	190 ± 12^a
GUO + LPS	112 ± 12^b	110 ± 10^b
ADO 100 + LPS	271 ± 15^a	182 ± 12^a
ADO 1000 + LPS	277 ± 21^a	185 ± 15^a
CAF 100 + LPS	283 ± 22^a	180 ± 14^a
CAF 1000 + LPS	268 ± 22^a	178 ± 15^a
GUO + CAF 100 + LPS	115 ± 14^b	107 ± 10^b
GUO + CAF 1000 + LPS	114 ± 12^b	115 ± 15^b

Cells were incubated in serum-free DMEM/F12 with adenosine (100 and 1000 μM) or 100 μM guanosine (GUO) and caffeine (100 and 1000 μM) for 1 h before being treated with 10 μg/mL LPS for 3 h. TNF-α and IL-1β levels were measured as described in the “Materials and methods” section. Data represent the mean ± S.E.M. of three independent experiments that were performed in triplicate. Differences between groups were statistically analyzed using two-way ANOVA, followed by Tukey’s test. Values of P < 0.05 were considered significant

^aDifferences from control conditions

^bDifferences from LPS