FIGURE 3.
A, RAW264.7 cells were cultivated overnight without stimulus ± inhibitors (MM, GI, and GW at 3 μm, recombinant pro-ADAM10 domain 10 μm) and sIL-6R was measured by ELISA. B, C57BL/6 BMDMs cells were treated as RAW264.7 cells in A and sIL-6R was quantified by ELISA. C, BMDMs from ADAM10Δmyeloid mice (n = 2 mice per group) were cultivated overnight without stimulus ± inhibitors (MM, GW 3 μm) and sIL-6R was measured by ELISA. Error bars represent S.D., n = 2 independent experiments. A and B, error bars are S.D., n = 3 independent experiments. ***, p < 0.001. veh. = dimethyl sulfoxide.