FIGURE 7.
IFITM1, IFITM2, and IFITM3 require palmitoylation for anti-HCV activity. A, schematic representation of IFITM1, IFITM2, and IFITM3 and the palmitoylation mutant derivatives. B, Huh-7 cells were transfected with wild-type IFITM proteins, the palmitoylation mutants, and vector control (empty) for 24 h prior to infection with HCV Jc1 (m.o.i. 0.003). Total RNA was harvested 24 h post-infection for RT-quantitative PCR for HCV RNA levels (data represented as a mean ± S.E. with a significance of **, p < 0.001; ***, p = 0.003; ****, p < 0.0001 calculated using a Student's t test).