Table 1.
Tyrosine kinases identified by phosphoproteomics in sarcoma cell lines
| A204 | RD18 | MNNG | U2OS | Saos2 | MG63 | A673 | RD-ES | SK-LMS1 | HT1080 | |
|---|---|---|---|---|---|---|---|---|---|---|
| ABL | 6 | 11 | 8 | 16 | 4 | 2 | 7 | 3 | 5 | 11 |
| ACK | 15 | 11 | 4 | 7 | 24 | 15 | 3 | 3 | 8 | 15 |
| ALK | 1 | 1 | ||||||||
| CSK | 3 | |||||||||
| DDR1 | 1 | 12 | 8 | |||||||
| DDR2 | 13 | 3 | 1 | 2 | 2 | 6 | 8 | 2 | 2 | |
| EGFR | 3 | 11 | 6 | 3 | 10 | 9 | 15 | 12 | ||
| EphA2 | 34 | 18 | 10 | 12 | 23 | 13 | 23 | 5 | 11 | 22 |
| EphA3 | 1 | 4 | 4 | 4 | 15 | 4 | 3 | 4 | ||
| EphA4 | 7 | 8 | 4 | |||||||
| EphA5 | 18 | 13 | 6 | |||||||
| EphA7 | 4 | 17 | 2 | 2 | ||||||
| EphB1 | 7 | 4 | 2 | 2 | 3 | 2 | ||||
| EphB2 | 16 | 3 | 6 | |||||||
| EphB3 | 4 | 10 | 9 | 6 | 3 | |||||
| EphB4 | 11 | 5 | 5 | 12 | 1 | 15 | 4 | 2 | ||
| FAK | 19 | 11 | 25 | 28 | 32 | 28 | 39 | 2 | 7 | 14 |
| FER | 2 | 3 | 2 | 4 | ||||||
| FGFR1 | 28 | 8 | 4 | 11 | 8 | 24 | 6 | 3 | 13 | |
| FGFR2 | 2 | 3 | ||||||||
| FGFR3 | 1 | |||||||||
| FGFR4 | 3 | 4 | 6 | |||||||
| FGR | 1 | 1 | ||||||||
| SRC:FYN/HCK | 24 | 17 | 14 | 17 | 10 | 15 | 21 | 17 | 10 | 17 |
| IGF1R | 2 | 9 | 8 | 10 | 5 | 5 | 2 | 1 | 6 | |
| INSR | 1 | |||||||||
| JAK1 | 3 | 4 | 2 | 1 | 5 | 1 | 7 | 4 | ||
| JAK2 | 11 | 3 | 3 | 4 | 1 | 5 | 4 | 4 | ||
| JAK3 | 1 | 1 | 1 | 2 | ||||||
| KDR | 1 | |||||||||
| KIT | 3 | 1 | ||||||||
| MER | 6 | 2 | 2 | 4 | 4 | 11 | 4 | 3 | 3 | |
| MET | 14 | 23 | 7 | 20 | 8 | 22 | 15 | |||
| MUSK | 4 | |||||||||
| PDGFRα | 33 | 10 | 1 | 10 | 23 | 13 | ||||
| RET | 14 | 1 | ||||||||
| TEC | 2 | 1 | ||||||||
| TYK2 | 4 | 4 | 2 | 3 | 5 | 2 | 3 | 4 | 3 | 3 |
| UFO/AXL | 6 | 4 | 5 | 13 | 19 | 24 | 3 | 13 | 15 |
NOTE: Ten sarcoma cell pellets were lysed and phosphopeptides were purified and identified by phosphopeptide immunoprecipitation and LC/MS-MS. pY sites associated with the corresponding tyrosine kinases were summed and total spectral counts from one biological sample and 2 MS-MS runs are listed.