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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Nat Cell Biol. 2014 Jan 7;16(2):191–198. doi: 10.1038/ncb2902

Figure 3. Mettl3 or Mettl14 knockdown mESCs lose self-renewal capability.

Figure 3

A. Phase contrast microscopy showing colony morphology of kd versus control mESC cells. Scr: scramble; shM3, shRNA against Mettl3; shM14, shRNA against Mettl14. B. Growth curve assessing cell proliferation kinetics of kd versus control cells. P values are generated by two-way ANOVA. C. Quantification of AP-positive colonies. D. Heat map analysis based on microarray comparison of gene expression in kd and control cells. E. RT-qPCR analysis of pluripotency (left) and differentiation (right) genes in kd versus control cells. F. GSEA analysis on enrichment of developmental regulators (left) and pluripotency-related genes (right) in Mettl3 kd versus control cells. A False Discovery Rate (FDR) of <0.178 was calculated for bivalent genes and FDR<0 for pluripotency-related genes. Note that FDR<0.25 is statistically significant for GSEA analysis: www.broadinstitute.org/gsea/doc/GSEAUserGuideFrame.html. Scale bars, 100 μm. Error bars from panels B-C and E represent mean ± SEM from 3 separate experiments. One-tailed Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P<0.0001 vs. scramble control. Scr: scramble.