Table 3.
Effects of miRNAs in lung epithelial cells.
| S. NO | miRNA | Cell type | Species | Regulation on notable target genes and validation method | Putative role | Reference |
|---|---|---|---|---|---|---|
| 1 | let-7d | A549, RLE-6TN and NHBE | Human and rat | ↓ HMGA2 (qRT-PCR) | Attenuates EMT | Pandit et al., 2010 |
| 2 | miR-21 | Primary alveolar type-2 epithelial cells | Mouse | ↑ Zeb1 and Zeb2 (qRT-PCR) | Promotes EMT | Yamada et al., 2013 |
| 3 | miR-200 | RLE-6TN | Rat | ↓ Zeb1, Zeb2 and Gata3 (qRT-PCR) | Attenuates EMT | Yang et al., 2012 |
| 4 | miR-29b | A549 | Human | ↓ Col1αl and Col3αl (qRT-PCR) | Attenuates EMT | Montgomery et al., 2014 |
| 5 | miR-326 | A549 and NHBE cells | Human | ↓ TGF-β1 (qRT-PCR and ELISA) | Attenuates EMT | Das et al., 2014 |
| 6 | miR-26a | A549 and mice lungs | Human and mouse | ↓ HMGA2 (Western blot and luciferase assay) | Attenuates EMT | Liang et al., 2014a |
| 7 | miR-27b | A549 | Human | ↓ Gremlin 1 (qRT-PCR and western blot) | Attenuates EMT | Graham et al., 2014 |
| 8 | miR-98 | A549 | Human | ↓ pStat3 and Stat3 (Western blot) | Attenuates EMT | Gao et al., 2014 |
| 9 | miR-424 | A549 | Human | ↓ Smurf2 (Western blot) | Promotes EMT | Xiao et al., 2015 |