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. 2015 Nov 3;5:16185. doi: 10.1038/srep16185

Figure 2. Ailanthone induces G0/G1 cell cycle arrest in Huh7 cells.

Figure 2

(A) Cell cycle profiles were measured by flow cytometry following treatment of the cells with various concentrations of ailanthone (0, 0.2, 0.4 or 0.8 μM) for 48 h or for various times (0, 36 h, 48 h, or 60 h) with 0.4 μM ailanthone. (B) Huh7 cells were treated with various concentrations of ailanthone or for various times. Western blotting was performed to detect p21, p27, cyclin D, cyclin E, CDK2, CDK4, CDK6, CDC25A and Rb. β-actin was used as a loading control.