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. Author manuscript; available in PMC: 2016 Jul 6.
Published in final edited form as: Dev Cell. 2015 Jun 25;34(1):58–72. doi: 10.1016/j.devcel.2015.05.016

Figure 7. The Regulation of Usp7 on Hh Signaling Is Evolutionally Conserved in Mammalian Cells and Zebrafish.

Figure 7

(A) Gli-luciferase (Gli-luc) reporter assay in 293T cells transfected with indicated constructs. Gli luciferase activities were normalized to Renilla Luciferase activities. The expression of indicated constructs was shown.

(B) Gli-luc reporter assay in 293T cells transfected with indicated siRNAs. Knockdown of hausp decreased Hh pathway activity. The protein levels of HAUSP and Gli were tested through western blotting analysis. Actin acts as a loading control.

(C-D) Gli-luc reporter assay in 3T3 transfected with indicated constructs.

(E) Gli-luc reporter assay in Sufu−/− MEFs transfected with indicated constructs. Of note, HAUSP regulated Hh pathway downstream of Sufu.

(F-I’) Expression of Hh target genes in zebrafish embryos that were injected with the indicated MOs at 10hpf (F-F’) or 24hpf (G-I’). Changes of the in situ staining are marked by arrows.

(J) Relative mRNA levels of zusp7, ptch2, fkd4, nkx2.2b and hhip from zebrafish embryos indicated in F-I’ were revealed by real-time PCR.

See also Figure S6.