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. 2014 Jun 26;5(6):e1308. doi: 10.1038/cddis.2014.257

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Corticosterone repressed MSCs differentiation into MFs. Mice serum was collected and analyzed for ALT (a) and AST (b). Sirius Red staining ( × 100) (c) and liver hydroxyproline analysis (d) were done to detect liver fibrosis. Hematoxylin eosin (HE) staining was done to detect liver injury ( × 200) (e). MSCs were induced to differentiate into MFs in vitro. MFs differentiation efficiency of MSCs was determined by cytoimmunofluorescence assay (f). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain nuclei (blue). OO, olive oil; CM, CCl₄+MSCs group; CMS, CCl₄+MSCs+stress group; CMSRU, CCl₄+MSCs+stress+RU486 group; CMCor, CCl₄+MSCs+corticosterone group; N, serum from normal mice; S, serum from stressed mice. RU, RU486; Cor, corticosterone. *P<0.05, **P<0.01