Figure 2.
The overexpression of miR-424 suppresses the apoptosis of tumor cells induced by anti-cancer chemicals. (a and b) Cleaved PARP and cleaved caspase-3 were detected using western blot analysis in HCT116 and A375 cells, which were transfected with a scrambled oligo or a miRNA-424 mimic. The final concentration of doxorubicin was 1 nM. (c and d) The annexin V-positive population of HCT116 or A375 cells was analyzed using flow cytometry. The final concentration of etoposide was 100 μM or 5 μM. (e) Cleaved PARP and cleaved caspase-3 levels were detected using western blot analysis in A375 cells that were transfected with a scrambled oligo or the miRNA-424 inhibitor. (f and g) The annexin V-positive populations of HCT116 or A375 cells were analyzed using flow cytometry. The final concentration of etoposide was 100 μM and 5 μM, respectively. (h) The survival ratio was analyzed using the CCK8 assay in HCT116 cells treated with doxorubicin (10 nM). HCT116 cells were transfected with the miRNA-424 mimic or inhibitor