TABLE 1.
Bacterial strains, plasmids, and cell lines used in this study
| Strain, plasmid, or cell line | Genotype or relevant characteristic(s) | Reference or source |
|---|---|---|
| S. aureus strains | ||
| RN6390 | 8325-4 wild type | 22 |
| QT1 | 8325-4 mgrA::cat or ΔmgrA | 25 |
| QT5 | 8325-4 norB::cat | 12 |
| QT7 | 8325-4 tet38::cat or Δtet38 | 12 |
| QT21 | 8325-4 ΔtetR21 | This study |
| QT22 | QT7(pLI50-tet38) | This study |
| QT23 | 8325-4 tet38::cat ΔtetR21 | This study |
| RN6390(pLI50-tet38) | tet38 overexpressor | This study |
| QT1(pLI50-mgrA) | Complementing QT1 strain | This study |
| QT21(pLI50-tetR21) | Complementing QT21 strain | This study |
| MW2 | CA-MRSA (USA400 lineage) | 11 |
| MW2ΔtetR21 | CA-MRSA mutant, ΔtetR21 | This study |
| MW2Δtet38 | CA-MRSA mutant, Δtet38 | This study |
| E. coli strains | ||
| BL21(DE3) | E. coli B F− dcm ompT hsdS(rB− mB−) gal λ(DE3) | Stratagene |
| Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(Strr) endA1 λ− | Invitrogen |
| DH10B | E. coli for cloning | 23 |
| BL21(DE3)(pTrcHisA-mgrA) | MgrA overexpressor | 25 |
| BL21(DE3)(pTrcHisA-tetR21) | TetR21 overexpressor | This study |
| Plasmids | ||
| pTrcHisA | Cloning and His tag-expressing vector in E. coli | Invitrogen |
| pIMAY | E. coli/S. aureus temp-sensitive plasmid | 23 |
| Cell lines | ||
| A549 | ATCC CCL-185, human lung adenocarcinoma | ATCC |
| HMEC-1 | Immortalized human dermal microvascular endothelial cell line | 27 |