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. Author manuscript; available in PMC: 2015 Oct 2.
Published in final edited form as: Cell Stem Cell. 2009 Sep 4;5(3):298–309. doi: 10.1016/j.stem.2009.06.001

Figure 4. Effects of Tie-2 Silencing and Tie-2 Overexpression on Mpcs.

Figure 4

(A) Mpcs were incubated with Tie-2 siRNAs and scrambled control siRNA (S) and analyzed. (Aa) Tie-2, Pax7, p130, MyoD, and p57 expression were evaluated by RT-qPCR. (Ab) Mpc proliferation and differentiation were evaluated after Ki67 and myogenin/desmin immunolabeling, respectively.

(B) Mpcs were incubated with Tie-2-overexpressing plasmid and analyzed. (Ba) Pax7, p130, MyoD, and p57 expression were evaluated by RT-qPCR. (Bb) Mpc proliferation and differentiation were evaluated as in (Ab). Results are means ± SEM.