FIGURE 2.
Identification of key amyloidogenic regions of ataxin-3. A, peptide scanning of the Josephin domain with aggregation monitored by ThT fluorescence. B–D, TEM of fibrils formed by peptides 76–90 (B), 146–160 (C), and 153–167 (D). E, peptide scanning of Josephin domain residues 73–102. F and G, TEM of fibrils formed by peptides 76–81 (F) and 91–96 (G). H, graphic representation of the Josephin domain solution structure with key amyloidogenic regions mapped onto the structure (Protein Data Bank (PDB) code 1YZB (22)). Scale bar equals 100 nm.