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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Immunity. 2015 Sep 1;43(3):451–462. doi: 10.1016/j.immuni.2015.08.008

Figure 6. NLRP3 and caspase-2 are required for caspase-1 activation during general ER stress.

Figure 6

(A) ELISA analysis of IL-1β in supernatants from BMDM treated with 200 ng/mL LPS and 1mM ATP; positive control for inflammasome activation), or the ER stressors BFA (brefeldin A, 20 μM), TM (tunicamycin, 10 μg/mL), and TG (thapsigargin, 10 μM). Error bars represent mean ± SD of n≥3 independent experiments. ** and *** represent p-values of <0.001 and <0.0001 respectively. n.s. = not significant. Immunoblot analysis of caspase-1 in (B) BMDM in the absence or presence of 4μ8c (IRE1 inhibitor, 50 μM), (C) WT and Nlrp3−/− BMDM – L+A╪ identifies duplicate lanes of the same sample, (D) WT and Casp2−/− BMDM, and (E) WT and Bid− /− BMDM. Immunoblots in (B–E) are representative of n≥3 independent experiments that were performed and imaged in parallel with identical parameters using a LiCor Odyssey imaging system. Full-length (FL) caspase-1 serves as a loading control. See also Supplemental Figure S6.