Fig 1.
mTORC1 inhibition induces eIF2α phosphorylation. A. Cell lines were treated with 100nM rapamycin (Rap) or tunicamycin (Tm) for six hours and eIF2α phosphorylation was assessed by immunoblot. Representative blots are displayed and quantitations reflect average and standard error (N=3 biological replicates). B. Primary MEFs and BJhTERT cells were treated with rapamycin for the indicated times or tunicamycin and eIF2α phosphorylation was assessed. Quantitation reflects average and standard error (N=3 biological replicates). C. U2OS cells were treated with rapamycin, tunicamycin, or depleted of rictor or raptor, and eIF2α phosphorylation was assessed. Representative blots are displayed and quantitation reflects average values (N=2 biological replicates).