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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: J Immunol. 2015 Aug 3;195(6):2908–2916. doi: 10.4049/jimmunol.1402974

Figure 3. Inhibition of Hedgehog signaling in WM cells has no effect on IgM secretion.

Figure 3

(A) mRNA expression of HH signaling components PTCH and SMO in BCWM.1, MWCL-1, and RPCI-WM1 cells determined by RT-PCR. (B) Effect of Cyclopamine (10 µM, 48 h) treatment on IgM secretion measured by ELISA. GLI1 expression was determined by RT-PCR. (C) Effect of Cyclopamine (10 µM, 48 h) treatment on relative proliferation of BCWM.1, MWCL-1, and RPCI-WM1 cells when compared with cells treated with DMSO control by XTT assay. (D) Effect of Cyclopamine (10 µM, 48 h) or DMSO control on relative viability of BCWM.1, MWCL-1, and RPCI-WM1 cells. Cells were analyzed by Annexin V/PI staining. Each experiment was repeated at least 3 times and the bars represent the average of 3-4 pooled biological replicates.