Figure 4.
Activation of CD28 enhances AMG 330-induced cytotoxicity in human AML cell lines. Parental CD33+ AML cell lines (ML-1 and TF-1) were incubated in the presence of AMG 330 (50 pg/ml) and healthy donor T-cells at an E/T cell ratio of 1:1 in the presence or absence of an activating CD28 antibody clone (CD28.2). After 48 h, cell counts were determined and cytotoxicity was assessed with DAPI staining to quantify drug-specific cytotoxicity. Results (mean±s.e.m.) are shown as from three independent experiments performed in duplicate wells using a single healthy donor as source for exogenous T-cells for determination of specific cytotoxicity.