Figure 2. MK1775 leads to apoptotic cell death in sarcoma cells.
A. MG63, U2OS, A673 and HT1080 cells were treated with 500nM MK1775 for 24 hours and the percentage of apoptotic cells was determined by Annexin V APC binding. Lower right quadrant of plots shows the number of cells in early apoptosis; these numbers were used in the bar graph. Upper right quadrant shows cells in late cell death. In the lower panels, MK1775 treatment results in multinucleation in sarcoma cells treated with 500nM MK1775 for 24 hours and multinucleation and micronucleation was observed using DAPI staining of nuclei and visualized using a Zeiss Upright Fluorescence microscope at 630×. B. Fold change Annexin V positive cells in control versus 500nM MK1775 treated cells for 24 hours.