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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Immunol. 2015 Jul 27;16(9):980–990. doi: 10.1038/ni.3226

Figure 4. Both TCF-1 and LEF-1 contribute to regulation of TFH differentiation and B cell responses.

Figure 4

(a) Flow cytometry of Tcf7-GFP expression in SMARTA CD4+ T cells (CD45.2+CD4+) at 8 d after Tcf7GFP/+ SMARTA cell transfer into CD45.1+ recipients and LCMV infection. Gated populations of TH1 (CXCR5−SLAMhi) and TFH (CXCR5+SLAMlo) cells were analyzed for Tcf7-GFP expression. Numbers indicate percent Tcf7-GFP+ cells. Data are representative of ≥ 2 experiments. (b-f) Flow cytometry of Tcf7−/−Lef1−/−Tcf7−/−, and littermate controls 8 d after infection i.v. with vaccinia virus. (b-d) Abundance of SLAMloCXCR5+ TFH cells (b), Bcl6+CXCR5+ GC TFH cells (c), and PD-1hiCXCR5+ GC TFH cells (d) gated on CD44hiCD62L−GFP+CD4+ T cells in spleen. (e-f) Abundance of GL7+Fas+ GC B cells (e) and IgDloCD138+ plasma cells (f) in the same animals (mean ± s.d.). Cumulative data from ≥ 3 experiments are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t-test).