Figure 4.
Analyses of amyloid metabolism-related indexes in the hippocampus. (a-b) Brain sections from isolated aged APP/PS1 mice and group controls were stained with Thioflavin-S (a) or immunostained with anti-6E10 antibody (b) to label plaques. Scale bar=500 μm. (c-d) Percentage of brain area occupied by Thioflavin-S (c) or 6E10 (d) labeled Aβ deposition in the hippocampus. (e-f) Western blotting and densitometry analysis of APP and its hydrolysis products, Aβ42 and sAPPα, and hydrolytic enzymes a disintegrin and metalloproteinase (ADAM) family protease ADAM10, β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) and PS1 as well as Aβ-degrading enzymes IDE and NEP. (g-h) Western blotting using 6E10 and densitometry analysis showed expression levels of APP, sAPPa, CTFβ, Aβ40, and Aβ42. Data represent means±SEM from 5 mice per group for pathological analyses and from 3 mice per group in 3 independent Western blotting analysis experiments. *P < .05, socially isolated (SI) aged APP/PS1 mice vs group (GR) controls.