Skip to main content
. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Clin Cancer Res. 2015 Apr 13;21(16):3727–3739. doi: 10.1158/1078-0432.CCR-14-2824

Figure 4. Curative effect of radiation requires CD4 and CD8 T cells, as well as cross-presenting dendritic cells, and is mediated by IFN-γ.

Figure 4

A, Survival of tumor-bearing animals after 30 Gy LTI depends on CD4+ and CD8+ T cells. Wild-type animals with 21 day CT26 tumors received 30Gy LTI (n=14) and anti-CD8 (n=8) or CD4 (n=5) depleting antibodies. B, Curative effect of LTI depends on CD8+ dendritic cells. Survival after single dose of 30 Gy LTI at day 21 in Batf3−/− mice with (n=9) and without (n=10) add back of purified CD8+ CD11c+ spleen cells is shown. The latter cells (5×106) were injected intratumorally within 1 day after irradiation. C, Cell subsets (CD11b+Gr-1hi, CD11b+Gr-1lo, CD11c, CD4+ and CD8+) are shown as a mean percentage +/− SE among mononuclear cells in tumor at day 14 after LTI in wild-type mice with or without CD4+ (n=5) or CD8+ T cells (n=5) depletion, in Batf3−/− mice with (n=5) and without (n=5) add back of CD8+ CD11c+ cells, and IFN-γ−/− mice (n=5). D, IFN-γ is necessary for curative effect of LTI. IFN-γ−/− (n=13), perforin−/− (n=5) and TNF-α −/− (n=5), and TLR4−/− (n=14) tumor-bearing mice were used. Survival after single dose of LTI at day 21 is shown.