Table 1.
Protein Candidates from LC-MS/MS Spot Analysis
| Spot No. | Gene Symbol | Protein Name | Fold Change by Maternal Smoking | Fold Change by Sex in Controls | Smoke Effect on Sex Differences (Fold Change) |
|---|---|---|---|---|---|
| 743 | USP5 | Ubiquitin carboxyl-terminal hydrolase 5 | +1.22 (♀) | No | No |
| HSP90AA1 | Heat shock protein 90 isoform 2 | ||||
| 990 | AFP | α fetoprotein | −1.31 (♀) | +1.15 (♀) | RSD;+1.20 (♂) |
| 1006 | SDHA | Succinate dehydrogenase | −1.23 (♀) | +1.26 (♀) | No |
| 1090a | CCT6A | T-complex protein 1 subunit zeta isoform a | No | +1.15 (♂) | No |
| 1123 | CAT | Catalase | +1.14 (♀) | No | ISD;+1.13 (♀) |
| 1129 | GLUD1 | Glutamate dehydrogenase 1, mitochondrial precursor | +1.18 (f) | No | ISD;+1.23 (♀) |
| CAT | Catalase | ||||
| 1149 | PDIA3 | Protein disulphide isomerase A3 | +1.21 (♂) | No | ISD;+1.21 (♂) |
| 1150a | SERPINA1 | α-1-antitrypsin precursor | +1.23 (♂) | No | ISD;+1.28 (♂) |
| 1172 | CALR | Calreticulin precursor | +1.30 (♀) | No | ISD;+1.14 (♀) |
| 1190 | KRT8 | Keratin type II Cytoskeletal 8 isoform 2 | +1.30 (♀) | No | ISD;+1.16 (♀) |
| 1205 | KRT8 | Keratin type II cytoskeletal 8 isoform 2 | +1.13 (♂) | No | ISD;+1.24 (♂) |
| 1221a | ALDH7A1 | α-aminoadipic semialdehyde dehydrogenase isoform 2 | −1.22 (♀); +1.24 (♂) | +1.27 (♀) | RSD;+1.26 (♂) |
| 1328 | PGK1 | Phosphoglycerate kinase 1 | +1.27 (♀) | No | ISD;+1.40 (♀) |
| 1501a | CRYL1 | Lambda crystallin homolog | +1.25 (♀) | +1.16 (♂) | No |
| 1589 | ECHS1 | Enoyl coenzyme hydratase 1 | +1.28 (♀) −1.33 (♂) | No | ISD; +1.42 (♀) |
| 1591 | PNP | Purine nucleoside phosphorylase | +1.24 (m) | No | ISD;+1.13 (♂) |
| 1645 | EEF1B2 | Elongation factor 1 β | +1.20 (♂) | No | ISD;+1.16 (♂) |
| YWHAE | 14-3-3 protein ϵ | ||||
| 1673 | ERP29 | Endoplasmic reticulum resident protein 29 isoform 1 precursor | No | No | ISD;+1.13 (♀) |
| 1678 | ERP29 | Endoplasmic reticulum resident protein 29 isoform 1 precursor | +1.16 (♀); −1.18 (♂) | +1.13 (♂) | RSD;+1.18 (♀) |
| TPI1 | Triose phosphate isomerase isoform 1 | ||||
| 1679 | HSPB1 | Heat shock protein β 1 | +1.18 (♂) | No | No |
| 1684 | ERP29 | Endoplasmic reticulum resident protein 29 isoform 1 precursor | +1.14 (♀) | No | ISD;+1.16 (♀) |
| 1786 | SPRYD4 | SPRY domain-containing protein 4 | +1.35 (♂) | No | ISD;+1.22 (♂) |
| FTL1 | Ferritin light chain |
Abbreviations: ISD, induction of sex difference; RSD, reversal of sex difference.
Only fold changes that achieved significance (P < .05) are shown.
a
Detection of a single candidate.