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. 2015 Aug 10;5:11754. doi: 10.1038/srep11754

Figure 6. Overexpression of STIM1 and/or ORAI1 in DU145 and PC3 cells remodels the tumor microenvironment.

Figure 6

A. Transwell assay of the migration of U937 cells after incubation with the conditioned medium (CM) from DU145 and PC3 cells in the low chamber for 12 h; representative crystal violet staining of U937 cells that migrated and attached to the bottom of the transwell filter are shown. B. Statistical analysis of violet staining intensity, measured as the absorbance at 560 nm (n = 3). C. Real-time RT-PCR analysis of mRNA expression changes of MMP9, VEGFA, IL10, IL6 and CD163 in U937 cells after treatment with the CM of DU145 and PC3 for 48 h (n = 3). D. STIM1 regulates the expression of cytokines in DU145 cells. Upper panel: representative results of cytokine arrays for DU145-YFP and DU145-STIM1-YFP. Lower panel: Statistical analysis of the relevant protein expression level of cytokines in DU145 cells. E. Real-time RT-PCR analysis of mRNA expression changes of IL8, MIF, CD54, CXCL1, IFNG, IL23A and SERPINE1 in DU145 and PC3 cells with STIM1-YFP and /or ORAI1 overexpression in comparison with those in control cells. F. Dual luciferase assay for NF-κB-RE activation in DU145 and PC3 cells.