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. 2015 Aug 10;5:11754. doi: 10.1038/srep11754

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Copyright © 2015, Macmillan Publishers Limited

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A. Western blotting analysis of STIM1 and ORAI1 in BPH-1, LNCaP, DU145 and PC3 cells. B. Comparison of SOCE activity in BPH-1, LNCaP, DU145 and PC3 cells. C. Statistical results of SOCE activities in human prostate cancer cells, represented as increases in the F₃₄₀/F₃₈₀ ratio over baseline values (ΔF₃₄₀/F₃₈₀) after extracellular Ca²⁺ application. D. Western blotting was used to confirm overexpression of STIM1 and/or ORAI1 genes in DU145 and PC3 cells. E. SOCE activities in DU145 cells with STIM1-YFP and /or ORAI1 overexpression and the DU145-YFP control. F. Increased SOCE activity in DU145-STIM1-YFP and DU145 -ORAI1-STIM1-YFP cells and slightly increased SOCE activity in DU145-ORAI1 cells when compared with that of DU145-YFP control.