TABLE 1.
Bacterial strains and plasmids used in this study
| Name | Description | Source or reference |
|---|---|---|
| Strains | ||
| S. marcescens | ||
| Db10 | Wild type | 41 |
| SJC3 | Db10 ΔtssH (ΔSMDB11_2274) | 7 |
| SJC11 | Db10 ΔtssE (ΔSMDB11_2271) | 7 |
| MJF15 | Db10 Δrhs1 (ΔSMDB11_2278) | This study |
| JAD13 | Db10 Δrhs2 (ΔSMDB11_1610) | This study |
| JAD14 | Db10 Δrhs1 Δrhs2 (ΔSMDB11_2278 ΔSMDB11_1610) | This study |
| JAD03 | Db10 ΔrhsI1 ΔtssH (ΔSMDB11_2278A ΔSMDB11_2274) | This study |
| JAD16 | Db10 Δrhs2 ΔrhsI2 (ΔSMDB11_1610 ΔSMDB11_1611) | This study |
| JAD12 | Db10 ΔeagR1 (ΔSMDB11_2277) | This study |
| JAD09 | Smr derivative of JAD03 (ΔrhsI1 ΔtssH) | This study |
| JAD17 | Smr derivative of JAD16 (Δrhs2 ΔrhsI2) | This study |
| JAD06 | Smr derivative of JAD01 (Δssp4 Δsip4) | 22 |
| SJC17 | Smr derivative of S. marcescens ATCC 274 | 7 |
| SM39 | Wild-type strain (intrinsically Smr) | N. Gotoh |
| Enterobacter cloacae ATCC 13047 | Wild-type strain (intrinsically Smr) | ATCC |
| Pseudomonas fluorescens KT02 | Smr derivative of P. fluorescens 55 | 7 |
| Escherichia coli | ||
| MC4100 | Model K-12 strain; Smr (rpsL150) | 42 |
| MG1655 | Wild type (model K-12 strain) | 43 |
| BL21(DE3) pLysS | Protein overexpression host carrying the λDE3 lysogen, allowing IPTG-inducible expression of T7 RNA polymerase, and pLysS, inhibiting basal levels of T7 polymerase in the absence of induction | 21 |
| CC118λpir | Cloning host and donor strain for pKNG101-derived allelic exchange plasmids (λpir) | 44 |
| HH26 pNJ5000 | Mobilizing strain for conjugal transfer | 45 |
| Plasmids | ||
| pBAD18-Kan | Arabinose-inducible expression vector (Kmr); gene of interest is cloned downstream of the Para promoter | 35 |
| pET15b-TEV | Protein overexpression vector for T7 polymerase-dependent expression of recombinant proteins, derived from pET15b | 21 |
| pKNG101 | Suicide vector for marker exchange (Smr sacBR mobRK2 oriR6K) | 46 |
| pSC635 | Coding sequence for Rhs1-CT (SMDB11_2278; amino acids 1333 to 1473) in pBAD18-Kan | This study |
| pSC636 | Coding sequence for RhsI1 (SMDB11_2278A) in pBAD18-Kan | This study |
| pSC637 | Coding sequences for Rhs1-CT plus RhsI1 in pBAD18-Kan | This study |
| pSC640 | Coding sequence for RhsI1 with a C-terminal HA tag in pBAD18-Kan | This study |
| pSC643 | Coding sequence for full-length Rhs1 (SMDB11_2278) in pBAD18-Kan | This study |
| pSC658 | Coding sequence for EagR1 (SMDB11_2277) in pBAD18-Kan | This study |
| pSC672 | Coding sequence for RhsI2 (SMDB11_1611) in pBAD18-Kan | This study |
| pSC673 | Coding sequences for RhsI1 and RhsI2 in pBAD18-Kan | This study |
| pSC674 | Coding sequence for Rhs2-CT (SMDB11_1610; amino acids 1290 to 1430) in pET15b-TEV (no affinity tag fused to Rhs2-CT) | This study |
| pSC675 | Coding sequences for Rhs2-CT plus RhsI2 in pET15b-TEV (no affinity tag fused to either protein) | This study |
| pSC619 | pKNG101-derived allelic exchange plasmid for the generation of chromosomal in-frame ΔSMDB11_2278A (ΔrhsI1) deletion | This study |
| pSC649 | pKNG101-derived allelic exchange plasmid for the generation of chromosomal in-frame ΔSMDB11_2277 (ΔeagR1) deletion | This study |
| pSC650 | pKNG101-derived allelic exchange plasmid for the generation of chromosomal in-frame ΔSMDB11_1610 (Δrhs2) deletion | This study |
| pSC664 | pKNG101-derived allelic exchange plasmid for the generation of chromosomal in-frame ΔSMDB11_1610-1611 (Δrhs2 ΔrhsI2) deletion | This study |
| pSC827 | pKNG101-derived allelic exchange plasmid for the generation of chromosomal in-frame ΔSMDB11_2278 (Δrhs1) deletion | This study |