Figure 2.
Optokinetic reflex behavior in wild type and valentino larvae. A) Quantitative and qualitative analyses of OKR. A) Eye position records were acquired and differentiated into velocity. Fast phase velocity was measured in low gain sweeps. Traces were desaccaded12 (dashed bracket) and the slow phase velocity (expanded view) divided into direct and indirect components to quantify performance. B) OKR can be separated into slow and fast phases that involve specific sets of neurons. Schematic illustrating the circuits for slow and fast phases. Refer to Supplementary Fig. 2 for the detailed wiring of the circuits. C) Table summarizing eye movements observed in val-/- with the neurons in r5-6 highlighted. D-E) Histograms showing the performance of Slow (D) and Fast phases (E) in val-/- when compared to wild type. Wild type n: Slow phase (direct = 713; indirect = 716), fast phase (nasal = 950; temporal = 939). Val-/- n: Slow phase (direct = 972; indirect = 1047), fast phase (nasal = 790; temporal = 987), Error bars (SEM). F-G) Representative OKR responses for one velocity step cycle of ±20°/s and H) ±5°/s at 0.0625 Hz in wild type F) and val-/- (G-H). Blue and brown lines represent mean velocity of direct and indirect components, respectively. Abd, Abducens motoneurons. Int, Internuclear neurons. nIII, Oculomotor nucleus. N, nasal. Pret, Pretectum. T, temporal. Vest(H), Horizontal Vestibular Neurons.