Extended Data Figure 7. HFSCs activate different epicenters within super-enhancers to sustain expression of critical genes in different microenvironments.
a, b, H3K27ac and HFSC-TF ChIP-seq occupancies at the Macf1 and Rad51b loci in HFSCs in vivo and in vitro. Regions C, E and F mark epicenters active in vivo, richly bound by HFSC-TFs; adjacent regions D and G are novel epicenters active in vitro. Relative luciferase activities were driven by the 1-1.5kb encompassing these epicenters. Mean and standard deviation are shown (n = 3). P-values from t-test: ***P<0.001. Functional validation of epicenter shifts in vivo. eGFP-reporter activity of in vitro epicenters is highly active in the epidermis while physiological HFSC epicenters are restricted to the HF niche. c, Motif analysis of Macf1 epicenters (Regions A and B, Fig. 3e) for putative TF binding sites. d, Number and distribution of HFSC-SE epicenters in vitro. e, Frequency of epicenter shifts in HFSC-SEs (in vivo versus in vitro). Note that corresponding to the loss of HFSC-TFs in vitro, many SEs display epicenter shifts to maintain expression of critical genes (e.g. Macf1) in different microenvironments.