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. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Vaccine. 2007 Jan 30;25(16):2984–2989. doi: 10.1016/j.vaccine.2007.01.063

Figure 4.

Figure 4

Interferon-γ ELISpot. Balb/c mice were immunized two weeks apart with 50ug pVAX vector or pH5HA and sacrificed one week later. Splenocytes were harvested and cultured overnight in the presence of R10 (negative control) or 10μg/mL of one of four peptide pools, made up of 15-mer peptides overlapping by 11 amino acids, spanning the length of the hemmaglutinin antigen. Spot forming units were quantified by an automated ELISPOT reader, and the raw values were normalized to SFU per million splenocytes. Values represent the mean (±SD) of duplicate wells.