Figure 2.
Diet-induced obesity in female ARKO mice. Female control and ARKO mice on apoE-deficient background were fed either an NCD or an HFD from 8 to 16 weeks of age. A) Body weights during the study. Lean mass (B), fat mass (C), and body fat percentage (D) analyzed by dual energy X-ray absorptiometry at 15 weeks of age (control NCD, n = 7; ARKO NCD, n = 7; control HFD, n = 12; ARKO HFD, n = 11). Weights of dissected visceral mesenteric (E) and subcutaneous inguinal fat depots (F) normalized to body weight (BW) at 16 weeks of age. (G) Body weight of female control (n = 9) and ARKO (n = 12) mice on an apoE-deficient background that were ovariectomized (OVX) before puberty and fed HFD from 8 to 16 weeks of age. Number of mice in each analysis (unless otherwise stated): control NCD, n = 13; ARKO NCD, n = 11; control HFD, n = 21; ARKO HFD, n = 17. All values are provided as means ± sem. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control HFD (Mann-Whitney test).