FIGURE 1.
A model containing autologous cells in subendothelial connective tissue. A, Low-power micrograph showing a cross-section of HUVECs grown on type I collagen with LCLs growing within the matrix. B, Higher power image shows the fragmenting nucleus of one LCL undergoing spontaneous death (arrow), surrounded by many living LCLs. C, Staining for propidium iodide and annexin V illustrates the degree of spontaneous apoptosis within LCLs and the extent to which it can be augmented by low dose gamma-irradiation. D, HA staining to monitor LCLs 16 h after infection with influenza A (P/R8) relative to staining with isotype-matched control mAb. Staining was done on nonpermeabilized (left) and permeabilized cells (right).