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. Author manuscript; available in PMC: 2016 Jun 10.
Published in final edited form as: Cell Host Microbe. 2015 May 28;17(6):788–798. doi: 10.1016/j.chom.2015.05.006

Figure 2. dacA Essentiality Assay.

Figure 2

(A) Schematic of dacA essentiality assay depicting erythromycin resistance genes (ermR), kana-mycin resistance genes (kanR) 3′ of dacA and ΔdacA, erythromycin (Erm)/kanamycin (Kan)-containing medium agar, and resistant trans-ductants (ermR/kanR). See text for description.

(B) Genetic linkage of dacA or ΔdacA with co-transduced antibiotic resistance marker in wild-type or mutants merodiploid for dacA (p-dacA).

(C) dacA essentiality assay of ΔdacA suppressor mutants, ΔdacA.3 (encoding a relAR295S mutation) was not determined due to an inability to obtain ermR transductants in this background. The relAR295S mutation was interrogated instead by reconstructing the mutation in a wild-type background. Dotted line indicates L.o.D., and all data are mean ± SEM of at least three independent experiments. See also Figure S1.