TABLE 1.
Oligonucleotides used in this study
| Use and primer | Sequence |
|---|---|
| qRT-PCR | |
| RNAIII-for | GTGATGGAAAATAGTTGATGAGTTGTTT |
| RNAIII-rev | GAATTTGTTCACTGTGTCGATAATCC |
| RNAIII probe | TGCACAAGATATCATTTCAACAATCAGTGACTTAGTAAAA |
| psmα-for | TATCAAAAGCTTAATCGAACAATTC |
| psmα-rev | CCCCTTCAAATAAGATGTTCATATC |
| psmα probe | AAAGACCTCCTTTGTTTGTTATGAAATCTTATTTACCAG |
| agrA-for | CGTAAGCATGACCCAGTTGGT |
| agrA-rev | CCATCGCTGCAACTTTGTAGAC |
| agrA probe | ATTATTTTCGTTACGAGTCACAGTGAACT |
| icaA-for | TGAACCGCTTGCCATGTG |
| icaA-rev | CACGCGTTGCTTCCAAAGA |
| icaA probe | TGGATGTTGGTTCCAGAAACATTGGGAG |
| clfA-for | AGGTTCTGGTGACGGTATCGA |
| clfA-rev | TCAATTTCACCAGGCTCATCAG |
| clfA probe | AAACCAGTTGTTCCTGAAC |
| clfB-for | TTCCAATGCGCAAGGAACTAG |
| clfB-rev | CAGCATTTACTACAGGTTCAGCAACT |
| clfB probe | AGACTACGTACAGCTCTCGTTCTAACACTT |
| fnbA-for | TGAGCCAGAAACTCCAACAC |
| fnbA-rev | TGGCAGGTGGTACTGGTTTA |
| fnbA probe | CGCCAACACCAGAGGTACCAGC |
| fnbB-for | AGGAATTAAGGCGGGAGATT |
| fnbB-rev | TGTCGCCATAACTTGACCAT |
| fnbB probe | CTCAACACTGCGTAAAGTTCCGGAGA |
| rsbU-for | ACCATAACGATGGCACAATG |
| rsbU -rev | TCCATAAGAATCCATGCCAA |
| rsbU probe | TCCCAATGACATCTGCAACAGCA |
| TRAP-for | CGTTTGTTAAGACCTGCTAAAGG |
| TRAP-rev | TGTCCGCTTGAACCAAAGTA |
| TRAP probe | TGCATGTCGATCAGCAAATCCG |
| Luciferase reporter gene fusion construction and verification | |
| luxBamHI | ATGCGGATCCTGCAGATGAAGCAAGAGGAG |
| luxSalI | ATGCGTCGACGCAGCGGTATTTTTCGATCA |
| P2prEco | ATGCGAATTCCTCATCAACTATTTTCCATCACATCTCTGT |
| P2prBam | CAATTTTACACCACTCTCCTCACTGGGATCCCATTATACG |
| P3prBam | ATGCGGATCCCTCATCAACTATTTTCCATCACATCTCTGT |
| P3prEco | CAATTTTACACCACTCTCCTCACTGGAATTCCATTATACG |
| psmβprSacI | GGCTTAGAAGGCCATTGCTGAGCTCAGCTGAGCTACCAGG |
| psmβprBam | TATCTTTAATTGCGTTAAATAAACCTTCCATTGAAAACAGGATCCAAAA |
| psmαprEco | GCCTAGACGAGACCTAACGTGGAATTCGTTTTAAAC |
| psmαprBam | GATGCCAGCGATGATACCCATTAAGATTGGATCCTTGCTTAT |
| scv4 | ACCCAGTTTGTAATTCCAGGAG |
| scv8 | GCACATAATTGCTCACAGCCA |
| scv9 | GCTGATCTAACAATCCAATCCA |
| scv10 | TATACCTCGATGATGTGCATAC |
| Cloning of psmβ operon | |
| Psmβ1Bam | CTTCAAATGGATCCTTTAAGGAGTGTTTTCAATGGAAGG |
| Psmβ2Mlu | CTGAGTGCATAACATACGCGTATGCTCACCCAGTTTATTTTAAAG |