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The slow proliferating fraction is appreciable also ex vivo in NSCLC PDX. Cells derived from disaggregation of PDX were PKH labeled and reinjected subcutaneously in SCID mice (n = 4). PKH retaining CD133+ enriched cells are appreciable in tumors analyzed at different volumes (V1 = 250 mm3, V2 = 1000 mm3) in both PDX tested; PDX111 (A) and PDX73 (B).
