Fig. 1.
Myeloid lineage knockout of Adam10 worsens clinical outcome in S. aureus skin infection. Abscess (a) and dermonecrosis (b) area in control and Adam10−/− mice following subcutaneous infection with 1 × 107 CFU S. aureus USA300/LAC. One representative experiment of 10 using 6-10 individual mice per group is shown. Lesion sizes were recorded by noninvasive measurements and the lesion area calculated according to the formula A = ([π/2] × l × w). * p < 0.05, Student's t test. c, d Mice were infected as in a and skin lesions were harvested 72 h (c) or 7 days (d) after infection with an 8-mm punch biopsy tool, then homogenized in PBS for quantification of tissue bacterial burden by serial dilution analysis. e Hla protein content in lesion homogenates 24 h after infection, measured by sandwich ELISA. f Gross pathologic image of control and Adam10−/− skin lesions 4 days after infection, where the abscess area (delineated by a dotted circle) surrounds the central dermonecrosis lesion. g HE-stained tissues of day 4 lesions in infected control and Adam10−/− mice. Black scale bar (low magnification images) = 500 μm; yellow scale bar (high magnification images) = 200 μm.