FIG. 1.
Double staining of lung tissue of a mouse using acid-fast staining followed by immunohistochemistry analysis with MAb 5c11. (A) Lung tissue of an uninfected mouse (original magnification, ×100); (B) lung tissue of a mouse infected with M. tuberculosis (original magnification, ×100) demonstrating areas of immunostaining (arrows); (C) lung tissue of a mouse infected with M. tuberculosis (bar, 10 μm) demonstrating immunostaining in dark blue; (D) acid-fast staining of lung tissue of a mouse infected with M. tuberculosis without the addition of MAb 5c11 (bar, 10 μm); panel D demonstrates an area equivalent to that shown in panel C following counterstaining with hematoxylin. The figure was generated with an Epson Perfection 1650 scanner and Adobe Photoshop (version 7.0) for the personal computer.