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. Author manuscript; available in PMC: 2016 Jun 2.
Published in final edited form as: Cell Metab. 2015 May 14;21(6):855–867. doi: 10.1016/j.cmet.2015.04.010

Figure 2. U17 snoRNA of Snhg3 locus complements the altered cholesterol trafficking in the I5 mutant.

Figure 2

(A) Structures of plasmid constructs for complementation are shown with exons as rectangles and snoRNAs as block arrows. (B) Sequences that are within U17 snoRNA m1/m2 elements are shown in gray boxes. Nucleotides mutated in the MUT+ construct shown in black. (C, D) Clonal lines expressing the various constructs were analyzed by qRT-PCR for expression of the murine U17 snoRNA (C) and Snhg3 mRNA (D), each normalized to β-actin. MUT+1 and MUT+2 are independent clonal lines expressing the MUT+ construct, in which both m1 and m2 elements are mutated. (E) Clones were assessed for esterification of PM-derived cholesterol. Data presented are mean + SE for 4 independent measurements. ***, ρ <0.001 for comparisons indicated