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. Author manuscript; available in PMC: 2016 Feb 10.
Published in final edited form as: J Control Release. 2014 Dec 3;199:156–167. doi: 10.1016/j.jconrel.2014.11.016

Figure 1. Purification and thermal characterization of LV96.

Figure 1

A) Cartoon of the LV96 fusion protein showing Lacrt at the N-terminus and an ELP tag at the C-terminus, with a thrombin recognition site between the two moieties. B) SDS-PAGE of purified LV96, ELP alone (V96) and free Lacrt. Gels were stained using CuCl2. C) Representative optical density profiles for LV96, V96 and Lacrt at 25 μM as a function of temperature, which indicate a phase separation at 26.8 (LV96) and 31.6°C (V96) respectively. Lacrt alone remains soluble, and does not increase optical density. D) A concentration temperature phase diagram was constructed. Best fit lines are indicated that follow Eq. 1 (Table 1).