FIG. 1.
Conservation of Oa1 regulatory region during evolution. (A) Sequence comparison between the murine and human Oa1 genomic regions upstream of the ATG codon (in bold). Bioinformatic analysis of the murine Oa1 promoter identified putative binding sites for known transcription factors. Binding sites that are conserved in the murine and human sequences are indicated. The large arrow indicates the transcription start site in the mouse gene. Open arrows indicate restriction sites used for the generation of construct pOa−562/+55. (B) The first 68 bp upstream of the ATG codon of the murine Oa1 gene were compared by Clustal methods to the same genomic region in the human, rat, and fugu genomes. Bases that are conserved in the mouse and other species are shaded. The E-box is boxed.