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. Author manuscript; available in PMC: 2016 May 19.
Published in final edited form as: Immunity. 2015 May 19;42(5):916–928. doi: 10.1016/j.immuni.2015.04.017

Figure 2. A discrete subset of migratory cDCs in skin-draining lymph nodes requires Klf4.

Figure 2

(A,B) Two-color histograms for CD11b and CD24 expression are gated on live cells for the resident (A) and migratory (B) gates shown in (S2A). (C) Single color histograms for intracellular IRF4 expression of the indicated subsets gated as in Figure 2A,B for resident and migratory DCs isolated from Klf4f/f cre-negative mice. Dotted line shows Irf4−/− control cDCs. Numbers represent geometrical mean values of IRF4 expression on the gated subsets. (D) Splenocytes from mice of the indicated genotypes were pre-gated as cDCs (MHC-IIhi CD11chi) and analyzed for CD24 and Sirp-α expression. Numbers indicate the percent of cells in the gate. (E and F) Two-color histograms for Sirp-α, CD24, CD11b, CD103 and Mgl2 as indicated in the plots, are shown for live cells from lung (E) and liver (F) of mice of the indicated genotype. Cells were pre-gated as CD45+ MHC-IIhi CD11c+ CD64−. Numbers indicate percent cells in the gates. All stainings were repeated in at least 3 independent experiments using at least 2 mice per genotype. See also Supplemental Figure S2